An important part of the nucleus is formed by nuclear lamina.
Nuclear lamins form a network of filaments at the nucleoplasmic site of the nuclear membrane.
Two main subtypes of nuclear lamins can be distinguished, i.e.
A type lamins and B type lamins.
The A type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e.
lamin A, lamin C and lamin Adel10, while the B-type lamins include two proteins arising from two distinct genes, i.e.
lamin B1 and lamin B2.
The nuclear lamins comprise a unique subclass of the intermediate filament protein family.
They share a molecular domain organisation with the other intermediate filament proteins in that they are fibrous molecules that have an aminoterminal globular head, a central rod of alpha helices and a carboxy terminal globular domain.
Many biochemical and molecular features of lamins have been studied, but their functions remain still largely undetermined.
One of the functions ascribed to the lamina is the maintenance of the structural integrity of the nucleus.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Lamin B1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 50-100 of Human Lamin B1.
