Kinase suppressor of Ras (Ksr) and MAP kinase kinase kinase (MEKK3) are integral members of the MAP kinase pathway.
Ksr is a conserved protein that positively regulates Ras signaling and may function as a scaffold for Raf, MEK and ERK.
There are two types of Ksr proteins: Ksr-1 and Ksr-2.
Ksr-2 plays a key role in Ras-mediated signaling during germline meiotic progression and functions redundantly with Ksr-1 during the development of the excretory system pathway, hermaphrodite vulva, and male spicules.
Ksr-2 also functions as a negative regulator of the MEKK3-mediated activation of the MAP kinase pathways (specifically ERK and JNK) and of the NF˚B pathways, and it simultaneously inhibits MEKK3-mediated Il-8 production.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Ksr2. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 662-724 of Human Ksr2.
