The kinesin superfamily proteins (KIFs) are microtubule-dependent molecular motors that transport membranous organelles and protein complexes in a microtubule- and ATP-dependent manner.
Cells use KIFs to tightly control the direction, destination and speed of transportation of a variety of important functional molecules, including mRNA.
KIF4A functions as an essential chromosome-associated molecular motor involved in faithful chromosome segregation.
It is found in the nucleoplasm during interphase and on condensed chromosome arms during mitosis.
KIF4A accumulates in the mid-zone duing late anaphase and on the cytokinetic ring during cytokinesis.
KIF4 binds to and translocates PRC1, a spindle mid-zone-associated cyclin-dependent kinase that plays a role in cytokinesis.
KIF4A may also interact with the condensin I and II complexes.
Loss of KIF4A leads to chromosome hypercondensation, suggesting that it is necessary for retaining normal chromosome architecture.
Applications:Suitable for use in Western Blot, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunocytochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of KIF4A. Species Crossreactivity: Human, mouse
Isotype：IgG
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to amino acids 1187-1232 of Human KIF4A.
