The kinesins constitute a large family of microtubule-dependent motor proteins, which are responsible for the distribution of numerous organelles, vesicles and macromolecular complexes throughout the cell.
Individual kinesin members play crucial roles in cell division, intracellular transport and membrane trafficking events including endocytosis and transcytosis.
KIF2B (kinesin family member 2B) is a 673 amino acid cytoplasmic protein that localizes to the kinetochore.
Highly expressed in lung and ovary with moderate expression in heart, kidney, placenta skeletal muscle and spleen, KIF2B has microtubule depolymerization activity and is associated with peripheral translocation of lysosomes.
Overexpression of KIF2B in cells result in abnormally large lysosome size and unusual positioning, which is some distance from their usual perinuclear location.
KIF2B activity is critical for spindle assembly and chromosome movement.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of KIF2B. Species Crossreactivity: Human
