84-2355-60　［取扱停止］KDM1A (Amine Oxidase (flavin Containing) Domain 2, Amine Oxidase flavin Containing Domain 2, Amine Oxidase flavin Containing Domain Protein 2, AOF 2, AOF2, BHC110, BRAF35 HDAC Complex Protein BHC110, BRAF35-HDAC Complex Protein BHC110, FAD Binding Protein BRAF35 HDAC Complex, 110 a Subunit, Flavin Containing amine Oxidase Domain Containing Protein 2, Flavin-Contai ...　223776

特徴

• Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context.
• Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed.
• Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation.
• Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me.
• May play a role in the repression of neuronal genes.
• Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity.
• Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.