Retention of resident soluble proteins in the lumen of the endoplasmic reticulum (ER) is achieved in both yeast and animal cells by their continual retrieval from the cis-Golgi, or a pre-Golgi compartment.
Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually lys-asp-glu-leu (KDEL) in animal cells, and his-asp-glu-leu (HDEL) in S.
cerevisiae.
This process is mediated by a receptor that recognizes, and binds the tetrapeptide-containing protein, and returns it to the ER.
In yeast, the sorting receptor encoded by a single gene, ERD2, which is a seven-transmembrane protein.
Unlike yeast, several human homologs of the ERD2 gene, constituting the KDEL receptor gene family, have been described.
The protein encoded by this gene was the first member of the family to be identified, and it encodes a protein structurally and functionally similar to the yeast ERD2 gene product.
Applications:Suitable for use in Immunofluorescence and, Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (Paraffin): 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, pH 7.2, 0.05% sodium azide
Specificity：Recognizes endogenous levels of human KDEL Receptor. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IF IHC
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 100-150 of human KDEL Receptor 2.
