c-Jun N-terminal kinases (JNKs) phosphorylate and augment transcriptional activity of c-Jun.
JNKs originate from three genes that yield 10 isoforms through alternative mRNA splicing, including JNK1a1,JNK1b1, JNK2a1, JNK2b1, and JNK3a1, which represent the p46 isoforms, and JNK1a2, JNK1b2, JNK2a2, JNK2b2, and JNK3b2, which represent the p54 isoforms.JNKs coordinate cell responses to stress and influence regulation of cell growth and transformation.
The human JNK1 (PRKM8, SAPK1, MAPK8) gene maps to chromosome 10q11.22 and shares 83% amino acid identity with JNK2.
JNK1 is necessary for normal activation and differentiation of CD4 helper T (TH) cells into TH1 and TH2 effector cells.
Capsaicin activates JNK1 and p38 in ras-transformed human breast epithelial cells.
Nitrogen oxides (NOx) upregulate JNK1 in addition to c-Fos, c-Jun, and other signaling kinases, including MEKK1 and p38.
JNK3 (MK10, MAPK10, PRKM10) is activated by pro-inflammatory cytokines and environmental stresss by phosphorylating transcription factors such as c-Jun and ATF2.
This is important for AP-1 transcriptional activity regulation.
JNK3 is crucial for neuronal apoptosis (stress-induced).
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Immunocytochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of JNK3 / MAPK10. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant full length Human JNK3.
Shelf Life：1year
Swiss Prot Number：P53779
EU Commodity Code：30021010
商品名：JNK3 / MAPK10 (c Jun Kinase 3, c-Jun N-Terminal Kinase 3, cJun N Terminal Kinase 3, FLJ12099, FLJ33785, JNK3 alpha Protein Kinase, JNK3, JNK3A, MAP Kinase 10, MAP Kinase, MAP Kinase p49 3F12, MAPK 10, Mapk10, MGC50974, Mitogen Activated Protein Kinase 10, Mitogen-Activated Protein Kinase 10, MK10, p493F12, p54bSAPK, PRKM10, Protein Kinase Mitogen Activated 10, SAPK1b, Stress Activated Protein Kinase 1b, stress Activated Protein Kinase beta, Stress Activated Protein Kinase JNK3, Stress-Activated Protein k) 50ul
