84-2354-97　［取扱停止］JIP-3 (C-jun-amino-Terminal Kinase-interacting Protein 3, JNK-interacting Protein 3, JIP-3, JNK MAP Kinase scaffold Protein 3, Mitogen-Activated Protein Kinase 8-interacting Protein 3, JIP3, KIAA1066) 50ul　223744

特徴

• c-Jun NH2-terminal kinases (JNKs) are distant members of the MAP kinase family.
• JNK1 is activated by dual phosphorylation at a Thr-Pro-Tyr motif in response to ultraviolet (UV) light, and it functions to phosphorylate c-Jun at amino terminal serine regulatory sites Ser 63 and Ser 73, resulting in transcriptional activation.
• Two additional JNK family members, JNK2 and JNK3, have been identified.
• JIP-1 (for JNK interacting protein-1) has been identified as a cytoplasmic inhibitor of JNK that retains JNK in the cytoplasm, thereby inhibiting JNK-regulated gene expression.
• Evidence suggests that JNK1 and JNK2 bind to JIP-1 with greater affinity than to ATF-2 and c-Jun, which are targets of the JNK signaling pathway.
• JIP-1 contains an amino terminal JNK binding domain and a carboxy terminal SH3 domain.
• ATF-2 and c-Jun also contain the JNK binding domain and are thought to compete with JIP-1 for JNK binding.
• Multiple splice variants of JIP-1, including JIP-1b, JIP-1c (also designated islet-brain 1 or IB-1), JIP-2a, JIP-2b and JIP-3, have been identified in brain.
• Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.