JNK/SAPK-inhibitory kinase (JIK) is a serine/threonine kinase that belongs to the STE20 kinase family.
The kinase domain of JIK is similar to the GCK-like subfamily of STE20 kinases, while its non-catalytic domain is similar to a Caenorhabditis elegans putative serine/threonine kinase, SULU.
JIK inhibits c-Jun NH2-terminal kinase/stress-activated protein kinase (JNK/SAPK), which is activated by many types of cellular stresses and extracellular signals.
JNK/SAPK regulates cell survival, apoptosis and proliferation in mouse development.
JIK is negatively regulated by epidermal growth factor (EGF) and tyrosine kinase receptors.
In unstimulated human T cells, JIK is cytoplasmic, whereas in the continuously dividing human T cells of Jurkat lymphoma, JIK is nuclear.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of JIK. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 540-589 of Human JIK.
