The inter-alpha trypsin inhibitor (ITI) family is a group of structurally related plasma serine protease inhibitors synthesized in the liver and built up from different combinations of three highly homologous heavy chains (ITI-HI, ITI-H2 and ITI-H3) and one light chain (bikunin).
A fourth member of the ITI family, ITI-H4 (also known as I alpha IH4P) harbours a Pro-rich region (PRR) in its c-terminus.
ITI (also known as IaI) is a 220kDa glycoprotein composed of three polypeptides linked by chondroitin sulphate: two heavy chains, ITI-H1 (65 kDa) and ITI-H2 (72 kDa), and bikunin (approx.
30 kDa).
Bikunin confers the protease-inhibitor function of ITI.
The heavy chains of the ITI family, designated as SHAPs (for serum-derived hyaluronan-associated proteins), bindcovalently to hyaluronic acid (HA), resulting in pericellular matrix stabilization.
ITI-H1 contains a potential peptide which could stimulate a broad spectrum of phagocytotic cells.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of ITI-H2. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 712-757 of Human ITI-H2.
