CENP-R (centromere protein R), also known as CENPR, ITGB3BP (Integrin beta3 binding protein), beta3-endonexin, NRIF3 (nuclear receptor-interacting factor 3) or TAP20, is a widely expressed protein that contains a DD1 or RepD1 domain and an LXXLL motif.
Induced by estrogen, CENP-R is believed to function as a nuclear receptor coactivator.
It also associates with the CENP-A-CAD complex which is involved in mitotic progression, the assembly of kinetochore proteins and chromosome segregation.
A number of isoforms exist for CENP-R due to alternative splicing events.
Isoform 1 localizes to the nucleus at the centromere; isoform 2, also known as EnL, En-L or long isoform, localizes to the nucleus; isoform 3, also known as EnS, En-S or short isoform, localizes predominantly to the nucleus but can also be found in the cytoplasm; and isoform 4 predominantly localizes to the cytoplasm.
Isoform 3 of CENP-R binds to the cytoplasmic tail of Integrin beta3 and can activate Integrin alphaIIb/beta3.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of ITGB3BP. Species Crossreactivity: Human
