Islet-1 (ISL1 transcription factor, LIM/homeodomain) and Islet-2 (ISL2 transcription factor, LIM/homeodomain) contain amino-terminal LIM domains and a carboxy-terminal homeodomain and both influence developmental events.
Islet-1 influences embryogenesis of the pancreatic islets of Langerhans and neural tube motor neuron differentiation.
In developing mouse teeth, Islet-1 mediates patterning of dentition as an activator of Bmp4 expression in incisor (distal) areas of the stomatodeal epithelium.
Islet-1 expression defines cardiac progenitor cell populations and is required for normal cardiac development and asymmetry.
Islet-2 activity in newly generated motor neurons permits the diversification of visceral and somatic motor neuron subtypes in the developing spinal cord.
Murine Islet-2 specifies retinal ganglion cell (RGC) laterality by repressing an ipsilateral pathfinding program unique to the ventral-temporal crescent (VTC) of RGCs in a Zic2- and EphB1-dependent manner.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of ISL1. Species Crossreactivity: Human, mouse, rat
