Interleukin-1 receptor (IL1R)-associated kinases (IRAKs) are important mediators in the signal transduction of Toll-like receptor (TLR) and IL1R family members, collectively referred to as TIRs.
IRAK family members include two active kinases, IRAK-1 and IRAK-4, and two inactive kinase, IRAK-2 and IRAK-M.
Binding of IL-1 to its cognate receptor results in the activation of the NFkB signaling pathway and MAP kinase pathways.
IRAK-4 appears to act upstream of other IRAKs and phosphorylates IRAK-1 on threonine 387.
It is highly expressed in liver and kidney tissues, but also displays a wide, low level of expression in other tissues.
IRAK-4 is an essential component of innate immunity.
Deficiency of IRAK-4 leads to recurrent bacterial infections and profound hyporesponsiveness to LPS and IL-1.
Therefore, IRAK-4 may be a potential target for therapeutic drug design.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of IRAK4. Species Crossreactivity: Human, mouse, rat
