84-2353-73　［取扱停止］IRAK-M (Interleukin-1 Receptor-Associated Kinase 3, IRAK-3, IL-1 Receptor-Associated Kinase M, IRAKM, IRAK3) 100ul　223682

特徴

• Interleukin-1 receptor (IL1R)-associated kinases (IRAKs) are important mediators in the signal transduction of Toll-like receptor (TLR) and IL1R family members, collectively referred to as TIRs.
• Binding of IL-1 to its cognate receptor results in the activation of the NFκB signaling pathway.
• A 100kDa IL-1- dependent kinase termed IRAK-1 (for IL-1 receptor- associated kinase 1) coimmunoprecipitates with activated IL-1RI and is implicated as an upstream mediator of NFκB activation.
• A related Drosophila protein, Pelle, is a known upstream activator of Dorsal, the Drosophila homolog of NFκB.
• IRAK-2 is a proximal mediator of IL1, a component of the IL1R signaling complex, and is required for IL1R-induced NFκB activation.
• IRAK-4, like IRAK-1 and Pelle, has auto- and cross-phosphorylation kinase activity.
• IRAK-4 is strongly expressed in kidney and is also found in lung, testis, small intestine, breast, liver, and placenta.
• In contrast to the other IRAKs that are expressed in most cell types, IRAK-M is restricted to monocytic cells.
• IRAK-M mRNA transcripts are found predominantly in PBL and the monocytic cell lines U937 and THP-1.
• Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.