Integrins are heterodimers composed of non-covalently associated transmembrane alpha and beta subunits.
The 16 alpha and 8 beta subunits heterodimerize to produce more than 20 different receptors.
Most integrin receptors bind ligands that are components of the extracellular matrix, including fibronectin, collagen and vitronectin.
Certain integrins can also bind to soluble ligands such as fibrinogen, or to counterreceptors on adjacent cells such as the intracellular adhesion molecules (ICAMs), leading to aggregation of cells.
Ligands serve to cross-link or cluster integrins by binding to adjacent integrin receptors; both receptor clustering and ligand occupancy are necessary for the activation of integrin-mediated responses.
In addition to mediating cell adhesion and cytoskeletal organization, integrins function as signaling receptors.
Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis.
The Integrin alpha3 chain, also known as very late (activation) antigen 3 (VLA-3), very common antigen 2 (VCA-2), extracellular matrix receptor 1 (ECMR1) and galactoprotein b3 (GAPB3), undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta1 to form an integrin that interacts with many extracellular-matrix proteins.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Integrin alpha3. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 500-550 of Human Integrin α3.
