INSIG-1 and INSIG-2 play distinct roles in a negative-feedback mechanism for cholesterol synthesis.
INSIG-1 localizes to the endoplasmic reticulum (ER) and binds the sterol-sensing domain of SREBP cleavage-activating protein (SCAP).
Sterol induces INSIG-1 binding to SCAP.
INSIG-2, another ER protein, binds SCAP in a sterol-regulated manner.
Thus, INSIG-1 and INSIG-2 block the export of SCAP from the ER and ultimately inhibit cholesterol synthesis by preventing the proteolytic processing of SREBPs by Golgi enzymes.
The critical role of INSIG-1 and INSIG-2 in cholesterol metabolism may be exploited as a therapeutic effect for hypercholesterolemia.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of INSIG2. Species Crossreactivity: Human, mouse, rat
