A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis.
The centromere proteins CENP-A, CENP-B, CENP-C, CENP-E, CENP-F (also designated mitosin), CENP-H and INCENP are kinetochore proteins that are involved in mitotic events.
The centromere proteins are expressed at different levels throughout the cell cycle and are involved in the formation of the centromere and the organization and function of the kinetochore.
INCENP, which also is designated inner centromere protein, is a chromosomal passenger protein that is crucial for chromosome segregation.
During mitosis it is also required for cytokinesis onset.
This protein, which can form a homodimer or a heterodimer, binds directly to microtubules and interacts with AURKB, AURKC, CBX3 and beta Tubulin.
This nuclear protein localizes to the mitotic spindle, methaphase chromosomes and during anaphase, to the equatorial cortex.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of INCENP. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human INCENP.
