ILKAP (integrin-linked kinase-associated serine/threonine phosphatase 2C),also known as PP2Cδ, is a 392 amino acid cytoplasmic protein phosphatase
that selectively interacts with integrin linked kinase (ILK) to regulate growth factor signaling and cell adhesion.
While widely expressed, ILKAP is found at highest levels in striated muscle with lower levels found in smooth muscle.ILKAP belongs to the PP2C family and contains one PP2C-like domain.
ILKAP has been suggested to inhibit oncogenic transformation and the ILK-GSK3betasignaling axis, and can bind two magnesium or manganese ions per subunit as cofactors.
The gene encoding ILKAP maps to human chromosome 2,which consists of 237 million bases encoding over 1,400 genes and making up ~8% of the human genome.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200 Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of ILKAP. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 40-90 of Human ILKAP.
