IL-6 activates intracellular signaling through binding a receptor consisting of a ligand-binding protein (IL-6Ralpha) and a second protein.
IL-6 first binds to IL-6Ralpha (also known as gp80), which subsequently associates with a gp130 dimer.
The active signaling complex consists of, at minimum, IL-6, IL-6Ralpha and a dimer of two gp130 proteins that are linked by a disulfide bond.
A soluble form of IL-6Ralpha, namely sIL-6Ralpha, is generated by proteolytic cleavage of the membrane-bound precursor and can function as an agonistic molecule that can actively participate in cell-to-cell signaling.
The second subunit of the IL-6 complex, gp130, also functions as a component of several additional receptor complexes, including leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF) and IL-11.
LIF binds to the LIF receptor with low affinity and to a complex of the LIF receptor and gp130 with high affinity, while OSM appears to bind to gp130 with low affinity and to a complex of gp130 and the LIF receptor with high affinity.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of IL6R. Species Crossreactivity: Human
