Four structurally related IL-1 receptor ligands have been described.
These include three agonists designated IL-1alpha, IL-1beta and IL-1y/IL-18 and a specific receptor antagonist, IL-1Ralpha.
IL-1alpha and IL-1beta play critical roles in the regulation of the immune response and inflammation, serving as activators of T and B lymphocytes and NK (natural killer) cells.
IL-18 (also referred to as IL-1y) has been shown to augment the secretion of IFN-y from T lymphocytes and increase NK cell activity in spleen cells.
IL-18 exhibits 19% and 12% identity with IL-1alpha and IL-1beta respectively over the 12 beta-strands of the beta-trefoil fold domain, which is a signature feature of the IL-1 family.
The unusual leader sequence of IL-18 may be analogous to the IL-1beta pro-domain which must be cleaved by the serine protease ICE for optimal secretion and biological activity.
Originally described as IGIF (IFN-y-inducing factor), IL-18 is induced by mouse liver subsequent to challenge with lipopolysaccharide (LPS).
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of IL18. Species Crossreactivity: Human
