IL-12, a heterodimeric cytokine composed of two disulfide-bonded glycoprotein subunits, p35 and p40, has pleiotrophic activities including stimulation of the proliferation of activated T and NK cells, induction of IFN-g production by PBMCs, enhancement of the lytic activity of NK/LAK cells and promotion of T-helper (Th) 1 cell responses.
The T cell response to IL-12 is mediated through two receptor proteins, designated IL-12Rb-1 and IL-12Rb-2 (4).
The genes encoding human IL-12Rb-1 and IL-12Rb-2 map to chromosomes 19p13.1 and 1p31.3-p31.2, respectively.
Increased IL-12Rb-2 expression is crucial in regulating Th1 differentiation, whereas IL-12Rb-1 expression is less restricted.
Inhibition of IL-12 activity may provide treatment for diseases associated with pathologic Th1 responses, such as multiple sclerosis or Crohn’s disease, while administration of recombinant IL-12 may aid in the treatment for allergic disorders and asthma.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of IL12RB1. Species Crossreactivity: Human
