Interleukin-3, or IL-3, is a pleiotropic cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells.
IL-3 exerts its biological effects through a receptor which consists of a ligand-specific alpha subunit (IL-3Ralpha) and a signal transducing beta subunit (IL-3Rbeta) common to the IL-3/IL-5/GM-CSF receptors.
The alpha subunits are low-affinity ligand-binding proteins while the beta subunits do not themselves bind ligand, but are required for high affinity binding by the alpha subunits.
The mouse IL-3 receptor has two distinct beta subunits, one that functions only in IL-3-mediated cell signaling and a second that is shared with IL-5 and GM-CSF.
The murine beta subunits are 91% homologous at the amino acid level but only 56% homologous to the human beta subunit.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of IL-3Rbeta. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human IL-3Rβ.
