Interleukin-32 (IL-32), also designated Natural Killer (NK) cell transcript 4 (nk4), may play a role in inflammatory responses such as lymphocyte activation.
IL-32 is secreted following stimulation with inflammatory cytokines such as IL-1beta and IFN-y, and by NK cells after exposure to IL-12 and IL-18.
It also induces production of IL-1beta, TNFalpha, MIP-2 and IL-6.
Expression of IL-32 increases after activation of T cells by mitogens or activation of NK cells by IL-2.
The IL-32 protein contains three potential N-myristoylation sites, a tyrosine sulfation site, an RGD cellattachment sequence and multiple putative phosphorylation sites.
Human IL-32 exists as four isoforms.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of IL-32. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 200-2450 of Human IL-32.
