IL-27 is a heterodimeric cytokine that consists of EBI3, an IL-12p40-related protein, and p28, a IL-12p35-related polypeptide.
IL-27 triggers expansion of antigen-specific naive CD4-positive T cells and promotes polarization towards a Th1 phenotype with expression of gamma-interferon.
IL-27 contributes to the development of an adaptive immune response through its action on CD4-positive T cells, and also directly acts on cells of the innate immune system.
IL-27 protein levels increase upon activation of antigen-presenting cells.
IL-27 protein induces orphan cytokine receptor IL-27R (WSX-1)-dependent clonal expansion of naive but not memory CD4(+) T cells.
IL-27 signaling through IL-27R and gp130 also induces phosphorylation of Stat1-5.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of IL-27Ralpha. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human IL-27Rα.
