Lymphokines are a group of signaling molecules involved in communication between cells of the immune system.
Lymphokines secreted by activated lymphocytes include proteins such as IL-2.
This protein is secreted primarily by helper T cells that have been activated through the T cell receptor complex or by other mitogens.
Cells targeted by IL-2 include activated T helper and cytotoxic T cells, inducing their proliferation.
The secretion of IL-2 can also act as a growth factor for B cells.
To date, three different IL-2-dependent signal transduction pathways have been identified: the c-Fos/c-Jun induction pathway mediated by Src family protein-tyrosine kinases; the c-Myc induction pathway; and the Rapamycin-sensitive pathway; all of which result in the induction of Bcl-2.
In addition, the transcription factor NFAT has been shown to play a major role in the regulation of IL-2 transcription and correlates to an age-related decline in the expression of IL-2.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-2000Immunohistochemistry: 1:50-200Immunocytochemistry: 1:50-200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of IL-2. Species Crossreactivity: Human
