Originally known as lymphocyte chemoattractant factor (LCF), Interleukin-16 (IL-16) is a product of human peripheral mononuclear cells when antigen-stimulated or mitogen-stimulated.
It is also produced by dendritic cells, epithelial cells, eosinophils, fibroblasts and mast cells.
The pro-form of IL-16 is a 631 amino acid protein that is cleaved by caspase 3 in activated CD4+ T cells where it is then secreted and is accessible for binding CD4 receptors.
IL-16 regulates and induces migratory response in CD4+ lymphocytes, eosinophils, and monocytes.
It is a soluble ligand for CD4 and functions to prime responsiveness to IL-2 and IL-15 in CD4+ T-cells.
Although Isoform 1’s function is thought to be as an anchor to membrane ion channels, Isoform 3 is more widely researched and characterized as component of T-cell cycle progression.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of IL-16. Species Crossreactivity: Human, mouse, rat
