IL-12 is secreted by macrophages and human B-lymphoblastoid cells in response to antigenic stimulation.
This IL is responsible for the differentiation of naive CD4+ T cells into type 1 helper T cells that produce interferon-y (IFN-y).
It also activates production of tumor necrosis factor alpha (TNFalpha) from T and natural killer (NK) cells, and it inhibits IL-4 mediated suppression of IFN-y.
IL-12 also has anti-angiogenic activity since the production of IFN-y increases the production of inducible protein-10 (IP-10).
IL-12 p40 represents a subunit of IL-12 that is induced by several pathogens.
IL-12 p70 is a subunit of IL-12 that is controlled through both the p35 and p40 genes in a cell type-specific fashion.
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (Paraffin): 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, pH 7.2, 0.05% sodium azide
Specificity：Recognizes endogenous levels of human IL-12A. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 32-81of Human IL-12A.
