Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degradation of IkB.
IkB kinase alpha (IKKalpha), previously designated CHUK, interacts with IkB-alpha and specifically phosphorylates I˚Balpha on Serine 32 and 36, the sites that trigger its degradation.
IKKalpha appears to be critical for NFkB activation in response to proinflammatory cytokines.
Phosphorylation of IkB by IKKalpha is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1.
The functional IKK complex contains three subunits, IKKalpha, IKKbeta and IKKγ (also designated NEMO), and each appear to make essential contributions to IkB phosphorylation.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of IKKγ. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to the N-terminual of Human IKKγ.
