The transcription factor NFκB is retained in the cytoplasm in an inactive form by the inhibitory protein IκB.
Activation of NFκB requires that IκB be phosphorylate on specific serine residues, which results in targeted degradation of IκB.
IκB kinase alpha (IKKalpha), previously designated CHUK, interacts with IκB-alpha and specifically phosphorylates IκB-alpha on Serines 32 and 36, the sites that trigger its degradation.
IKKalpha appears to be critical for NFκB activation in response to proinflammatory cytokines.
Phosphorylation of IκB by IKKalpha is stimulated by the NFκB inducing kinase (NIK), which itself is a central regulato for NFκB activation in response to TNF and IL-1.
The functional IKK complex contains three subunits, IKKalpha, IKKbeta and IKKγ (also designated NEMO), and each appear to make essential contributions to IκB phosphorylation.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of IKKbeta, phosphorylated Y188. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide derived from human IKKβ around the phosphorylation site of Tyrosine 188.
