The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins.The regulation of IκBbeta and IκBε is similar to that of IκBalpha.
However, the phosphorylation and ubiquitin-mediated degradation of these proteins occurs with much slower kinetics.
IKK phosphorylation of IκBbeta occurs at Ser19 and Ser23, while IκBε can be phosphorylated at Ser18 and Ser22 .
The human sequence of IκB-beta has also been reported to contain a threonine at position 19 suggesting that phosphorylation could be Thr19/Ser23.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 15mM sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of IkB-beta, phosphorylated S23. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide derived from human IkB-β around the phosphorylation site of Serine 23.
