The insulin-like growth factor-binding proteins, or IGFBPs, are a family of homologous proteins that have co-evolved with the IGFs.
They serve not only as shuttle molecules for the soluble IGFs, but also confer a level of regulation to the IGF signaling system.
Physical association of the IGFBPs with IGF influences the bio-availability of the growth factors, as well as their concentration and distribution in the extracellular environment.
In addition, the IGFBPs appear to have biological activity independent of the IGFs.
Seven IGFBPs have thus far been described, each differing in their tissue distribution, half-lives and modulation of IGF interactions with their receptors.
For instance, IGFBP1 is negatively regulated by insulin production.
The IGFBP1 gene is expressed at a high level during fetal liver development and in response to nutritional changes and diabetes.
It has been suggested that IGFBP2 functions as chaperone, escorting IGFs to their target tissues.
It is expressed in several human tissues including fetal eye and fetal brain.
IGFBP3 is the most abundant IGFBP and is complexed with roughly 80% of the serum IGFs.
Both IGFBP3 and IGFBP4 are released by dermal fibroblasts in response to incision injury.
IGFBP5 is secreted by myoblasts and may play a key role in muscle differentiation.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of IGFBP4. Species Crossreactivity: Human, mouse, rat
