84-2350-01　［取扱停止］IGF2BP1 (Insulin-like Growth Factor-II mRNA-Binding Proteins (IMPs) belong to a Family of zipcode-Binding Proteins. Three Members of this Family, IMP1, IMP2, and IMP3, have been identified. They contain two RNA recognition Motifs, four K Homology Domains, and were found to function in mRNA localization, turnover, and Translation Control. Research studies have impl ...　223492

特徴

• IGF-II mRNA-binding proteins (IMP) bind RNA and influence RNA synthesis and metabolism.
• IMPs, IMP-1 (coding region determinant-binding protein/insulin-like growth factor II mRNA-binding protein, CRD-BP, VICKZ1), IMP-2 (IMP2, VICKZ2, p62) and IMP-3 (KOC1, VICKZ3), contain a unique combination of RNA recognition motifs and four hnRNP K homology domains.
• IMP-1 is abundant in embryonal tissues and in 81% of colon cancers, 58.5% of breast cancers and 73% of sarcomas.
• IMP-1 recognizes c-Myc, IGF-II and tau mRNAs, and H19 RNA and plays a major role in proliferation of K-562 cells by an IGF-II-dependent mechanism.
• IMP-2 binds the 5' UTR of IGF-II mRNA and influences tumor cell growth, in which IMP-2 is associated with apoptosis induced by tretinoin.
• IMP-3 knock down by RNA interference decreases levels of IGF-II protein without affecting IGF-II, c-Myc, or b Actin mRNA and H19 RNA levels.
• IMP-3 is a marker for carcinomas and high-grade dysplastic lesions of pancreatic ductal epithelium.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.