The mannose 6-phosphate/insulin-like growth factor II receptor, IGF-IIR (also designated M6P/IGF2R), is an ubiquitously expressed integral glycoprotein.
By binding glycoproteins through two of its extracytoplasmic domains, IGF-IIR mediates the activation of TGFbeta1 (a growth inhibitor), the degradation of IGF-II and the transport of lysosomal enzymes.
Subsequently, IGF-IIR can form oligomeric complexes, which increase the affinity of IGF-IIR for lysosomal enzymes.
Unlike IGF-IR, IGF-IIR does not potentiate the signaling of IGF-I or IGF-II, which have mitogenic, cell survival and insulin-like effects.
Therefore,IGF-IIR is characterized as a tumor suppressor.
Furthermore, the IGF-IIR gene is located on chromosome 6q26, which is commonly mutated or deleted in several human cancers.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of IGF-2R. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 2441-2490 of Human IGF-2R.
