The insulin gene family, comprised of insulin, relaxin and insulin-like growth factors I and II (IGF-I and IGF-II), represents a group of structurally related polypeptides whose biological functions have diverged.
The IGFs, or somato-medins, constitute a class of polypeptides that have a key role in pre-adolescent mammalian growth.
IGF-I and -II are critical regulators of cell proliferation and differentiation.
Most of the growth promoting properties of both ligands are mediated by the IGF-I receptor (IGF-IR).
IGF- I and -II, respectively known as somatomedin C and somatomedin A, are single chain polypeptides which share an amino acid sequence homology of about 47% with insulin.
IGF-I expression is regulated by growth hormone and mediates postnatal growth, while IGF-II is induced by placental lactogen during prenatal development.
IGF-II is a fetal growth factor, influenced by placental lactogen and abundantly expressed by placental trophoblasts.
IGF-II and IGF-binding protein 1 (IGFBP1) gene variants are associated with overfeeding-induced metabolic changes.
The human IGF-II gene maps to chromosome 11p15.5, encoding a 180 amino acid protein which is the precursor to IGF-II.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of IGF-2. Species Crossreactivity: Human, mouse, rat
