Interferon (IFN)-y is an antiviral and antiparasitic agent produced by CD4+/CD8+ lymphocytes and natural killer cells that undergo activation by antigens, mitogens or alloantigens.
IFN-y production modulates T cell growth and differentiation and inhibits the growth of B cells.
Synthesis of IFN-y is inducible by IL-2, FGF and EGF.
The active form of IFN-y is a homodimer with each subunit containing six helices.
The dimeric structure of human IFN-y is stabilized by non-covalent interactions through the interface of the helices.
IFN-y tranlsated precursor is 166 amino acids, including the 23 amino acid secretory sequence.
Multiple forms exist due to variable glycosylation and under non-denaturing conditions due to dimers and tetramers.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of IFN-γ. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 31-80 of Human IFN-γ.
