84-2349-28　［取扱停止］Id4 (DNA-Binding Protein Inhibitor ID-4, Class B basic Helix-Loop-Helix Protein 27, bHLHb27, Inhibitor of DNA Binding 4, ID4, BHLHB27) 50ul　223456

特徴

• Members of the Id family of basic helix-loop-helix (bHLH) proteins include Id1 , Id2, Id3 and Id4.
• They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins.
• Due to the absence of the basic region, the resulting heterodimers cannot bind DNA.
• The Id-type proteins thus appear to negatively regulate DNA binding of bHLH proteins.
• Since Id1 inhibits DNA binding of E12 and Myo D, it apparently functions to inhibit muscle-specific gene expression.
• Under conditions that facilitate muscle cell differentiation, the Id protein levels fall, allowing E12 and/or E47 to form heterodimers with Myo D and myogenin, which in turn activate myogenic differentiation.
• It has been shown that expression of each of the Id proteins is strongly dependent on growth factor activation and that reduction of Id mRNA levels by antisense oligonucleotides leads to a delayed reentry of arrested cells into the cell cycle following growth factor stimulation.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.