The CED/ICE family of cysteine proteases plays a pivotal role in mediating apoptosis through the proteolysis of specific targets.
Among the targets are poly (ADP-ribose) polymerase (PARP), gelsolin, DFF-45/ICAD and the nuclear lamins.
PARP is a 112kDa nuclear protein that is specifically cleaved by CPP32 and Mch2, but not by ICE, into a signature 85kDa apoptotic fragment.
Gelsolin is cleaved by CPP32 to an active form that severs actin filaments in a Ca++-independent manner.
In addition to binding actin, gelsolin can form complexes with fibronectin, which may be important for localizing gelsolin to inflammatory sites.
DFF-45/ICAD, the 45kDa subunit of DNA fragmentation factor, is cleaved by CPP32 to generate an active factor that induces DNA fragmentation.
The 70kDa nuclear Lamin A is cleaved by Mch2, but not CPP32.
Nuclear Lamin B is fragmented as a consequence of apoptosis by an unidentified member of the ICE family.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of ICAD. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 151-200 of Human ICAD.
