The intestinal alkaline phosphatase gene (ALPI) encodes a digestive brush-border enzyme, IAP (also designated ALP), which is highly upregulated during small intestinal epithelial cell differentiation.
IAP, an enterocyte differentiation marker that functions to limit fat absorption, has been implicated in trans-cellular transport of chylomicrons and in chylomicron formation.
At high pH, IAP removes phosphate groups from proteins and from the 5' end of DNA and RNA.
Most mammals have four different IAP isozymes: placental, placental- like, intestinal and non tissue-specific.
Non tissue-specific isozymes are found in liver, kidney and bone.
Tissues with particularly high concentrations of IAP include the liver, bile ducts, placenta and bone.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of IAP. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human IAP.
