84-2348-87　［取扱停止］HTRA2 (High temperature requirement Protein a2, HTRA 2, HtrA like Serine Protease, HtrA Serine peptidase 2, HtrA2, HTRA2_HUMAN, Mitochondrial, Omi stress Regulated endoProtease, Omi stress-Regulated endoProtease, PARK 13, PARK13, Protease Serine 25, PRSS 25, PRSS25, Serine Protease 25, Serine Protease HTRA2, Serine Protease HTRA2 Mitochondrial, Serine Protease htr ...　223434

特徴

• The human homolog of the E.
• Coli htrA gene product, HtrA, is identified in osteoarthritic cartilage and is repressed in SV40-transformed fibroblast.
• The gene encoding HtrA protein is highly conserved among mammalian species and belongs to the serine protease family.
• The HtrA protein contains an IGF-binding domain and exhibits endoproteolytic activity, including autocatalytic cleavage.
• HtrA is a secreted protein that is expressed in heterologous systems.
• HtrA plays a role in the degradation of denatured proteins and cell growth regulation.
• Human HtrA2 (also designated Omi), a novel member of the HtrA serine protease family, is highly homologous to HtrA (also known as L56 and HtrA1).
• HtrA2 is a ubiquitously expressed nuclear protease that is capable of autoproteolysis.
• The HtrA2 protein exists as two polypeptides and as an alternatively spliced form called D-Omi, which is predominately expressed in the kidney, colon and thyroid.
• Due to a modified PDZ domain, D-Omi does not interact with the known partner of HtrA2, the Mxi2 protein.
• Like HtrA, HtrA2 is involved in the degradation aberrantly folded proteins during conditions of cellular stress, suggesting that it may possess a chaperone-like role under normal conditions.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.