84-2348-06　［取扱停止］HSF1 (Heat Shock Factor 1, Heat Shock Factor Protein 1, Heat Shock Transcription Factor 1, HSF 1, hsf1, HSF1, HSTF 1, HSTF1) 100ul　223388

特徴

• Heat shock transcription factors (HSF, also designated HSTF) 1 and 2 are involved in this regulation.
• HSF1 and HSF2 are upregulated by estrogen at both the mRNA and protein level.
• HSF1 is normally found as a monomer whose transcriptional activity is repressed by constitutive phosphorylation.
• Upon activation, HSF1 forms trimers, gains DNA binding activity and is translocated to the nucleus.
• HSF2 activity is associated with differentiation and development, and, like HSF1, binds DNA as a trimer.
• Both HSF1 and HSF2 are known to be induced by proteasome inhibitors of the ubiquitin pathway.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.