3 beta-hydroxysteroid dehydrogenase (3beta-HSD), also known as HSD3B1 or HSDB3, is a bifunctional enzyme that plays a crucial role in the synthesis of all classes of hormonal steroids.
Two human 3beta-HSD proteins, designated type I (3beta-HSD) and type II (3beta-HSD2), are expressed by different genes and function in different areas of the body.
Localized to the membrane of the endoplasmic reticulum (ER) and expressed in skin and placenta, 3beta-HSD is the type I protein that catalyzes the oxidative conversion of ∂5-ene-3-beta-hydroxy steroid, as well as the conversion of various ketosteroids.
Defects in the gene encoding 3beta-HSD are associated with classic salt wasting, genital ambiguity, hypogonadism, insulin-resistant polycystic ovary syndrome (PCOS) and an increased susceptibility to prostate cancer.
Additionally, congenital deficiency of 3beta-HSD activity results in a severe depletion of steroid formation which can be lethal in young children.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human HSD3B2. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human HSD3B2.
