Histamine is an inflammatory mediator that is ubiquitously expressed and has a broad range of pharmacologic effects.
Specifically, it plays a role in the central nervous, gastrointestinal, respiratory and immune systems.
The effects of histamine are mediated by a family of G protein coupled receptors, the histamine H1, H2, H3 and H4 receptors.
The gene encoding the human histamine H1 receptor maps to chromosome 3p25 and is expressed in highest abundance in placenta, with lower levels in lung, skeletal muscle, kidney and brain.
The murine histamine H2 receptor gene maps to chromosome 13 and is highly expressed in stomach with moderate expression in brain and heart.
The gene encoding the human histamine H3 receptor is located on chromosome 20 and is expressed as six alternative splice variants in thalamus.
Applications:Suitable for use in Immunofluorescence, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of HRH2. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to the 179-224 of Human HRH2.
