84-2347-73　［取扱停止］HRF (Translationally-Controlled Tumor Protein, TCTP, Fortilin, Histamine-releasing Factor, HRF, p23, TPT1) 100ul　223372

特徴

• Histamine is an inflammatory mediator that is ubiquitously expressed and has a broad range of pharmacologic effects.
• Specifically, it plays a role in the central nervous, gastrointestinal, respiratory and immune systems.
• Histamine release is mediated by the stimulation of mast cells and basophils.
• Histamine-releasing factor (HRF) is a cytokine-like molecule that causes the release of histamine, IL-4 and IL-13 from basophils as well as the secretion of IL-8 and a calcium response in eosinophils.
• HRF belongs to the translationally controlled tumor protein (TCTP) family and is detected as a protein of 21kDa in mice and 23kDa in human.
• It is expressed in several healthy and tumoral cells, including erythrocytes, hepatocytes, macrophages, platelets, keratinocytes, erythroleukemia cells, gliomas, melanomas, hepatoblastomas and lymphomas, and it is localized in the cytoplasm.
• HRF plays a pivotal role in allergic diseases, and due to its wide distribution in brain, is thought to be involved in neurodegenerative disorders, such as Alzheimer’s disease and Down Syndrome.
• Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.