Heparanases (HPA) degrade heparan sulfate side chains of heparan sulfate proteoglycans (HSPGs) in the extracellular matrix and play animportant role in the extravasation of blood-borne tumor cells and inflammatory leukocytes.
HPA1 dismantles the subendothelial basal membrane and facilitates the metastasis of blood-borne tumor cells.
Furthermore, HPA1 induces angiogenesis and likely promotes the vascularization of tumors.
Upon degradation, HPAs free growth factors and cytokines that stimulate cell proliferation and chemotaxis.
Fibroblasts endocytose extracellular HPA1 for cytoplasmic accumulation in vitro.
Proteolytic processing at the cell surface of a precursor begets an active form of HPA1.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of HPSE. Species Crossreactivity: Human
