Heme oxygenases are microsomal enzymes that cleave heme to produce the antioxidant biliverdin, inorganic iron and carbon monoxide (CO).
The activity of Heme Oxygenase 1 (HO-1), also designated HSP 32, is highly inducible in response to numerous stimuli, including heme, heavy metals, hormones and oxidative stress.
Heme Oxygenase 2, in contrast, appears to be constituitively expressed in mammalian tissues.
Heme Oxygenase 2 is involved in the production of carbon monoxide (CO) in brain, where CO is thought to act as a neurotransmitter.
The CO signaling system closely parallels the signaling pathway involving nitric oxide, and regulation of the two systems is closely linked.
Heme Oxygenase 3 is found in the spleen, liver, thymus, prostate, heart, kidney, brain and testis.
A poor heme catalyst, Heme Oxygenase 3 has two heme regulatory motifs that may be involved in heme binding.
Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Immunocytochemistry: 1:50-1:200Immunoprecipitation: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of HO-1. Species Crossreactivity: Human, mouse, rat
