Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of poly-peptides that contribute to mRNA transcription and pre-mRNA processing, as well as mature mRNA transport to the cytoplasm and translation.
They also bind heterogeneous nuclear RNA (hnRNA), which is the transcript produced by RNA polymerase II.
There are ~20 known hnRNP proteins, and their complexes are the major constituents of the spliceosome.
The majority of hnRNP protein components are localized to the nucleus; however, some shuttle between the nucleus and the cytoplasm.
Two abundant and ubiquitously expressed members of the hnRNP family are hnRNP D0 and hnRNP R.
Specifically, the hnRNP D0 protein contains two RNA-binding domains (RBDs), which bind to both RNA and DNA sequences.
hnRNP D0 also possesses a transactivator domain and is involved in transcriptional regulation.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of HNRNPR. Species Crossreactivity: Human, mouse, rat
