Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to mRNA transcription, pre-mRNA processing as well as mature mRNA transport to the cytoplasm and translation.
They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II.
There are ~20 known hnRNP proteins, and their complexes are the major constituents of the spliceosome.
The majority of hnRNP protein components are localized to the nucleus; however some shuttle between the nucleus and the cytoplasm, such as hnRNP E1 and E2.
hnRNP E1 may function in the cytoplasm as a translational regulatory protein, while hnRNP E2 stabilizes mRNA to enhance polioviral mRNA translation.
hnRNP M is involved in pre-mRNA splicing and in stress-induced transient splicing arrest.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of hnRNP E1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human hnRNP E1.
