HNF-1 (alpha and beta), HNF-3 (alpha, beta and y), HNF-4 (alpha and y), and HNF-6 compose, in part, a homoeprotein family designated the Hepatocyte Nuclear Factor family.
The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus.
HNF-3alpha, HNF-3beta and HNF-3y regulate the transcription of numerous hepatocyte genes in adult liver.
HNF-3alpha and HNF-3beta have also been shown to be involved in gastrulation events such as body axis formation.
HNF-4alpha and HNF-4y have been shown to be important for early embryo development.
HNF-4alpha is expressed in liver, kidney, pancreas, small intestine, testis and colon; and HNF-4y is expressed in each of these tissues except liver.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 15mM sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of HNF4alpha/γ. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 100-150 of Human HNF4α.
