The human enzyme hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) limits the rate of cholesterol synthesis, a necessary process for cellular growth, in liver tissue.
Phosphorylation of HMGCR inactivates the enzyme, which occurs via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from the product of the reductase reaction.
Inhibitors of HMGCR (statins) exert anti-inflammatory effects and decrease the frequency of cardiovascular events by lowering plasma cholesterol.
Additionally, intermediate products along the pathway catalyzed by HMGCR, which modulate signal transducing proteins such as Ras, provide possible ties between HMGCR regulation and new chemotherapeutic methods.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of HMGCR. Species Crossreactivity: Human, mouse, rat
