84-2346-57　［取扱停止］HMGA1 (High Mobility Group Protein HMG-I/HMG-Y, HMG-I(Y), High Mobility Group AT-hook Protein 1, High Mobility Group Protein A1, High Mobility Group Protein R, HMGA1, HMGIY) 50ul　223314

特徴

• High mobility group (HMG) chromatin proteins bind to the minor groove of AT-rich DNA sequences with high affinity.
• Evidence suggests that the binding of HMG proteins to DNA induces alterations in the DNA architecture including DNA bending and unwinding of the helix.
• HMG proteins synergize with Oct-2, members of the NFkB family, ATF-2 and c-Jun to activate transcription.
• Other studies indicate that phosphorylation of HMG protein is required to stimulate the transcriptional activity of the protein.
• Human HMG-I/HMG-y contains two DNA-binding domains, termed HMG boxes.
• HMG proteins bind single-stranded DNA but induce conformational changes in double-stranded DNA alone.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.